Tlr5 Knockin mouse models
Serving as the flagellin sensor, Tlr5 is the mouse ortholog of human TLR5 and encodes a cell-surface leucine-rich repeat receptor that recognizes the conserved D1 domain of bacterial flagellin through a lateral surface centered on repeats seven to ten, an interface that also mediates receptor dimerization and subsequent MyD88, IRAK4, and TRAF6 dependent NF-kB activation. Because this epitope is buried in assembled filaments, TLR5 detects monomeric flagellin released by motile bacteria, and signaling in intestinal epithelium and lamina propria dendritic cells controls flagellin-specific IgA, antimicrobial peptide expression, and microbiota composition, with human dominant-negative variants linked to Legionella susceptibility. Nulls are viable but develop altered microbiota, metabolic syndrome features on some backgrounds, and impaired mucosal antibody responses, so germline deletion is informative provided cohousing and microbiome controls are used, while conditional alleles resolve epithelial versus dendritic cell sensing. Align allele choice with colonization, adjuvant, or metabolic endpoints.
Tlr5 knockin models place a defined sequence at the endogenous locus. Expression stays under native regulation, which matters for reporters, tags, and precise allele swaps.
What Tlr5 Knockin mouse models are available?
ingenious targeting laboratory offers 2 distinct Tlr5 knockin catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Why this approach
Knockin and humanized formats keep regulatory context at the endogenous locus. For your target tissue focused programs, that matters when expression timing, splice isoforms, or allele dosage drive biology. Random integration transgenics can still help, but targeted alleles usually give cleaner pharmacology readouts.
Pricing and quotes
The Tlr5 Knockin lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Tlr5 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Tlr5 Knockin mouse models are available?
When Tlr5 Knockin lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Tlr5 knockout embryonic lethal in mice?
It depends on background and allele design. Some Tlr5 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Tlr5 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Tlr5?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.