Tlr7 Knockin mouse models
Tlr7 is the mouse ortholog of human TLR7 and encodes an endosomal receptor that requires UNC93B1 for trafficking and proteolytic maturation, then senses single-stranded RNA degradation products through two cooperating sites, one binding guanosine or synthetic agonists such as imiquimod and R848 and a second binding short uridine-containing oligoribonucleotides, with dimerization driving MyD88, IRAK4, and IRF7 dependent type I interferon in plasmacytoid dendritic cells. Dosage is central to disease logic: the Yaa locus duplication of Tlr7 accelerates murine lupus, human gain of function substitutions such as Y264H that increase guanosine affinity cause childhood systemic lupus erythematosus, and escape from X inactivation raises expression in females. Design accordingly, using conditional nulls to assign requirement to plasmacytoid dendritic cells or B cells, copy-number or point-mutant knockins for gain of function, and humanized alleles for agonist pharmacology. Tlr8 and Tlr9 overlap in nucleic acid sensing, so target autoantibody, interferon signature, or adjuvant endpoints.
Targeting Tlr7 preserves positional context compared with viral or BAC approaches. That helps when you quantify expression or map chromatin.
What Tlr7 Knockin mouse models are available?
ingenious targeting laboratory offers 1 distinct Tlr7 knockin catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Tlr7-Y264H | Knockin | point mutantion mice | live | KI 230071 | Inquire |
Why this approach
Knockin and humanized formats keep regulatory context at the endogenous locus. For your target tissue focused programs, that matters when expression timing, splice isoforms, or allele dosage drive biology. Random integration transgenics can still help, but targeted alleles usually give cleaner pharmacology readouts.
Pricing and quotes
The Tlr7 Knockin lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Tlr7 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Tlr7 Knockin mouse models are available?
When Tlr7 Knockin lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Tlr7 knockout embryonic lethal in mice?
It depends on background and allele design. Some Tlr7 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Tlr7 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Tlr7?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.