Tradd Knockout mouse models
As the mouse ortholog of human TRADD, Tradd encodes the TNFR1-associated death domain protein, the first adaptor recruited to the ligated TNFR1 death domain, using its own C-terminal death domain for receptor binding and an N-terminal TRAF-interacting domain to dock TRAF2, thereby delivering cIAP1 and cIAP2 and enabling the ubiquitin-dependent assembly that drives NF-kB and MAPK activation. In the alternative configuration TRADD nucleates cytosolic complex II with FADD and caspase-8 to execute apoptosis, and it also participates in DR3 and TLR-driven signaling. Nulls are viable and fertile, showing reduced TNF-induced NF-kB and marked resistance to TNF-dependent apoptosis while retaining RIPK1-dependent necroptosis, so germline deletion is an efficient epistasis tool and conditional alleles resolve hepatocyte or intestinal epithelial requirement. Because RIPK1 can be recruited independently of TRADD, compound Ripk1 genetics clarifies the split, and alleles should follow hepatotoxicity, colitis, or death pathway endpoints.
Whole body loss of Tradd gives the clearest readout when the question is whether the gene is required at all. Follow up tissue work can still move to a conditional allele if lethality or compensation appears.
What Tradd Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Tradd knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Tradd-KO | Knockout | KO/CKO mice | embryo cryopreservation | KO 191169 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Tradd Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Tradd allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Tradd Knockout mouse models are available?
When Tradd Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Tradd knockout embryonic lethal in mice?
It depends on background and allele design. Some Tradd germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Tradd animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Tradd?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.