Traf5 Knockout mouse models
Traf5, the mouse ortholog of human TRAF5, encodes the closest structural relative of TRAF2, sharing a RING domain, zinc fingers, and a trimerizing TRAF domain, and it is recruited to CD27, CD30, CD40, lymphotoxin beta receptor, and TNFR2 to support NF-kB and JNK activation, in some assays acting interchangeably with TRAF2 while in others tuning IL-6 driven STAT3 signaling and helper T cell differentiation. Because its contribution is largely additive rather than unique, germline nulls are viable and fertile with only subtle defects in CD27 and CD40 driven responses, which is precisely the redundancy trap to plan for: a single Traf5 knockout will usually not report pathway dependence, and compound Traf2 Traf5 conditional genetics is required to expose shared function. RING-dead knockins separate ligase activity from scaffolding within otherwise intact receptor complexes. Fit the allele set to T cell dependent antibody responses, non-canonical NF-kB activity, or airway inflammation endpoints.
A conventional Traf5 knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Traf5 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Traf5 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Traf5-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 220406 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Traf5 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Traf5 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Traf5 Knockout mouse models are available?
When Traf5 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Traf5 knockout embryonic lethal in mice?
It depends on background and allele design. Some Traf5 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Traf5 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Traf5?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.