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Recombination Systems & Tools

Inversion vs Excision (Recombination Outcomes)

The orientation of recombination sites determines genetic outcomes: direct repeats drive excision (deletion) of the intervening DNA, whereas inverted repeats cause inversion of the DNA segment.

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Overview

The orientation of recombination sites determines genetic outcomes: direct repeats drive excision (deletion) of the intervening DNA, whereas inverted repeats cause inversion of the DNA segment. This principle underlies most conditional strategies.

Frequently Asked Questions

How do I force excision rather than inversion?

Sites must be in the same orientation (direct repeats) and on the same DNA strand.

Is inversion reversible?

Yes. Inversions can revert if recombinase activity continues.

Related Services

Related Terms

Cre-lox System

A bacteriophage P1-derived site-specific recombination technology that utilizes Cre recombinase to mediate genomic modifications at engineered LoxP sequences. This technology underpins conditional gene engineering approaches in mammalian genetics.

Flp-FRT System

A yeast-derived site-specific recombination mechanism enabling targeted DNA excision, inversion, or integration through the interaction of Flp recombinase with FRT sites. It serves as an orthogonal complement to Cre-lox in mammalian genetics.

Floxed Gene / LoxP Site

A floxed gene contains two LoxP sites flanking an essential DNA segment, allowing Cre recombinase to mediate excision or inversion for conditional gene regulation. LoxP sites are 34 base pair sequences recognized by Cre recombinase.

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Cre-lox SystemCre Driver Line (Tissue-Specific Cre)Floxed Gene / LoxP SiteLox-STOP-Lox (LSL) CassetteFlp-FRT System
Inducible Cre (Cre-ERT2 / Tamoxifen)View All TermsKnockin (KI) Mouse Models

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