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Vectors, ES Cells & Delivery

Pronuclear Injection (Transgenics)

A technique used to generate transgenic mice by directly introducing foreign DNA into the pronucleus of a fertilized one-cell embryo. The injected DNA integrates randomly into the mouse genome.

Overview

Pronuclear injection is a technique used to generate transgenic mice by directly introducing foreign DNA into the pronucleus of a fertilized one-cell embryo. The injected DNA integrates randomly into the mouse genome, resulting in offspring that carry and express the transgene.

Frequently Asked Questions

What is the main limitation of pronuclear injection?

DNA integrates randomly, resulting in variable expression and copy number. This can lead to position effects and unpredictable phenotypes.

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Related Terms

Single-Copy Integration

The precise insertion of a single copy of a DNA construct into a defined location within the genome. This approach prevents the variability, gene silencing, and expression artifacts associated with random multi-copy insertions.

Safe-Harbor Targeted Transgenesis

A precise genome engineering strategy in which a transgene is inserted into a well-characterized, transcriptionally active, and non-disruptive genomic locus to ensure predictable and stable expression.

More in Vectors, ES Cells & Delivery

Targeting Vector (Homology Arms)Selection Markers (NeoR, PuroR)Blastocyst Injection / Chimera ProductionSingle-Copy IntegrationLarge-Fragment / BAC Targeting
Promoter / Enhancer / Regulatory ElementView All TermsRecombination Leakiness (Background Activity)

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