Overview
Recombination leakiness refers to unintended recombinase activity occurring without inducer presence or outside targeted tissues, leading to unwanted genomic alterations or mosaicism. Understanding its sources and mitigation strategies is essential for accurate genetic modeling.
Frequently Asked Questions
What causes recombination without inducer?
Basal promoter activity, ligand-independent nuclear entry, or high transgene copy number.
How can I detect leakiness early?
Include Cre-reporter crosses and vehicle-only controls; quantify background recombination by PCR or imaging.