Vectors, ES Cells & Delivery

Safe-Harbor Targeted Transgenesis

A precise genome engineering strategy in which a transgene is inserted into a well-characterized, transcriptionally active, and non-disruptive genomic locus to ensure predictable and stable expression.

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Overview

Safe-harbor targeted transgenesis is a precise genome engineering strategy in which a transgene is inserted into a well-characterized, transcriptionally active, and non-disruptive genomic locus—a 'safe harbor'—to ensure predictable and stable expression.

Frequently Asked Questions

What are the most common safe harbor loci?

The Rosa26 locus is the most widely used, followed by H11 and TIGRE, each characterized by neutrality and broad compatibility with diverse promoters.

Related Services

Safe Harbor Locus Targeting Rosa26 Targeting

Related Terms

Safe Harbor Locus

A defined region of the genome where a transgene or targeted DNA sequence can be inserted without disrupting endogenous genes or regulatory networks. Integration at a safe harbor site allows stable, predictable expression.

Rosa26 Locus

A well-characterized safe harbor site in the mouse genome, located on chromosome 6. It supports stable and ubiquitous expression of inserted genes without interfering with nearby genomic functions and is the standard integration site for transgenic constructs.

Single-Copy Integration

The precise insertion of a single copy of a DNA construct into a defined location within the genome. This approach prevents the variability, gene silencing, and expression artifacts associated with random multi-copy insertions.

More in Vectors, ES Cells & Delivery

Targeting Vector (Homology Arms)Selection Markers (NeoR, PuroR)Blastocyst Injection / Chimera Production Pronuclear Injection (Transgenics)Single-Copy Integration

Safe Harbor Locus View All Terms Sanger / NGS Validation of Junctions

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