Aldob Knockout mouse models
Hereditary fructose intolerance is fundamentally a story about Aldob, the mouse ortholog of human ALDOB, whose product is the liver, kidney cortex, and intestinal aldolase isoform distinguished by high catalytic efficiency toward fructose-1-phosphate rather than fructose-1,6-bisphosphate alone. When aldolase B activity fails, ketohexokinase continues phosphorylating dietary fructose, fructose-1-phosphate accumulates, phosphate and adenine nucleotides are sequestered, and hypoglycemia, urate elevation, hepatomegaly, and proximal tubular injury follow. That mechanism makes allele choice sharp: a conditional null answers whether hepatocyte fructolysis drives steatosis and lipogenesis, whereas knockin of a catalytically crippled patient substitution such as A149P reproduces misfolding with residual activity closer to clinical biochemistry. Aldoa and Aldoc cannot substitute, since their fructose-1-phosphate kinetics are poor, so redundancy is not the usual confounder here. Pair Alb-Cre or Villin-Cre with defined fructose challenge protocols and read plasma glucose, hepatic ATP and phosphate, uric acid, and triglyceride flux.
Aldob null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What Aldob Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Aldob knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Aldob-KO | Knockout | KO/CKO mice, disease model mice | sperm cryopreservation | KO 200602 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Aldob Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Aldob allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Aldob Knockout mouse models are available?
When Aldob Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Aldob knockout embryonic lethal in mice?
It depends on background and allele design. Some Aldob germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Aldob animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Aldob?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.