Cox15 Knockout mouse models
Cox15 encodes the mouse ortholog of human COX15, the inner membrane heme A synthase that completes cofactor maturation by hydroxylating and oxidizing a methyl group of heme O to yield heme A, the reaction immediately downstream of the farnesylation catalyzed by Cox10. Heme A is required for MT-CO1 maturation and for the heme a and heme a3 centers that drive electron transfer to oxygen, so blocking this step produces isolated cytochrome c oxidase deficiency with degradation of unassembled subunit 1. Human COX15 mutations cause fatal infantile hypertrophic cardiomyopathy and Leigh syndrome, phenotypes making cardiac and neuronal conditional alleles the most informative designs, while hypomorphic knockins model residual heme A synthesis. Cox10 works in the same pathway and cannot substitute for the synthase step. Use Myh6-MerCreMer or Nes-Cre with echocardiography, COX and SDH histochemistry, complex IV spectrophotometry, and blue native assembly profiling matched to the question.
Cox15 null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What Cox15 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Cox15 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Cox15-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 202035 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Cox15 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Cox15 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Cox15 Knockout mouse models are available?
When Cox15 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Cox15 knockout embryonic lethal in mice?
It depends on background and allele design. Some Cox15 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Cox15 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Cox15?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.