Cox4i2 Knockout mouse models
Cox4i2, ortholog of human COX4I2, encodes the alternative isoform of cytochrome c oxidase subunit 4 that predominates in lung, particularly in pulmonary artery smooth muscle, and lacks the ATP binding regulatory motif of the constitutive isoform, so complex IV containing it escapes allosteric inhibition by high ATP. Because this isoform is induced under hypoxia and has been implicated in hypoxic pulmonary vasoconstriction, it links oxygen sensing to contractile signaling as well as to electron transfer. Biallelic human variants cause exocrine pancreatic insufficiency with dyserythropoietic anemia and calvarial hyperostosis, so patient knockins address clinical fidelity while a conditional null tests the vasoconstriction hypothesis. Cox4i1 is co-expressed in many tissues and can partly substitute structurally, which may mask phenotypes, so consider compound designs. Use smooth muscle drivers such as Myh11-CreER with hypoxic pulmonary hypertension protocols, right heart hemodynamics, vasoreactivity, and complex IV activity as endpoints.
Global Cox4i2 deletion answers broad mechanism questions quickly. If timing or site matters, conditional alleles are a natural next step after the null is characterized.
What Cox4i2 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Cox4i2 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Cox4i2-KO | Knockout | KO/CKO mice | embryo cryopreservation | KO 201494 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Cox4i2 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Cox4i2 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Cox4i2 Knockout mouse models are available?
When Cox4i2 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Cox4i2 knockout embryonic lethal in mice?
It depends on background and allele design. Some Cox4i2 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Cox4i2 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Cox4i2?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.