Dlg1 Knockout mouse models
Dlg1, ortholog of human DLG1, encodes SAP97, a membrane-associated guanylate kinase built from three PDZ domains, an SH3 module and a catalytically inactive guanylate kinase domain, whose alternatively spliced N-terminal L27 or palmitoylated variants determine whether it acts in epithelial polarity with Scribble and Lgl or in neuronal receptor trafficking. In neurons it escorts GluA1 through the secretory pathway and binds Kv1.4 and the cardiac sodium channel Nav1.5, linking it to both synaptic delivery and excitability, while in epithelia it enforces basolateral identity and is a target of viral oncoproteins. Constitutive nulls die perinatally with cleft palate, urogenital and kidney defects, so a conditional null is mandatory for adult neural work; pair Camk2a-Cre for forebrain synapses or a cardiomyocyte driver for conduction endpoints. Dlg2, Dlg3 and Dlg4 share domain architecture and partially substitute at the postsynaptic density, so compound alleles and direct receptor surface measurements are advisable.
A conventional Dlg1 knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Dlg1 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Dlg1 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Dlg1-KO | Knockout | KO/CKO mice | embryo cryopreservation | KO 226254 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Dlg1 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Dlg1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Dlg1 Knockout mouse models are available?
When Dlg1 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Dlg1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Dlg1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Dlg1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Dlg1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.