Dlg4 Knockout mouse models
PSD-95, encoded by Dlg4 as the ortholog of human DLG4, is the dominant scaffold of the excitatory postsynaptic density, anchored by palmitoylation of Cys3 and Cys5 and organizing receptors through PDZ1 and PDZ2 binding to the GluN2 ESDV motif and to transmembrane AMPA receptor regulatory protein C-termini including γ-8, while PDZ2 recruits nNOS and the guanylate kinase domain assembles the GKAP, Shank and Homer layers. Its abundance sets AMPA receptor content per synapse, and disruption of the nNOS interaction is an established stroke neuroprotection strategy. Constitutive nulls are viable but show markedly enhanced long-term potentiation, absent long-term depression and impaired spatial learning, so lethality is not a constraint; a conditional null with Camk2a-Cre isolates forebrain contributions and a palmitoylation-site or PDZ-binding knockin separates membrane anchoring from protein interaction. Because Dlg1, Dlg2 and Dlg3 upregulate and partly substitute, compound conditional genetics and quantitative postsynaptic density proteomics should be planned from the start.
Whole body loss of Dlg4 gives the clearest readout when the question is whether the gene is required at all. Follow up tissue work can still move to a conditional allele if lethality or compensation appears.
What Dlg4 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Dlg4 (PSD-95) knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Dlg4-KO | Knockout | KO/CKO mice | embryo cryopreservation | KO 190951 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Dlg4 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Dlg4 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Dlg4 Knockout mouse models are available?
When Dlg4 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Dlg4 knockout embryonic lethal in mice?
It depends on background and allele design. Some Dlg4 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Dlg4 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Dlg4?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.