Ndufa4 Knockout mouse models
Despite its name, Ndufa4, the mouse ortholog of human NDUFA4, encodes a subunit reassigned from complex I to complex IV, where it binds the cytochrome c oxidase monomer and prevents dimerization, acting as a bona fide fourteenth COX subunit that modulates catalytic efficiency. Human biallelic NDUFA4 variants cause Leigh syndrome with isolated complex IV deficiency, consistent with that reassignment and providing a rare case in which the gene name actively misdirects experimental design. Allele planning should therefore be built around complex IV endpoints, with a conditional null using neuronal or cardiac Cre to test tissue thresholds for COX activity and patient variant knockins to model the hypomorphic human state. Importantly, the paralog Ndufa4l2 is induced by HIF1A in hypoxic and tumor tissue and substitutes at the same site with altered kinetics, so compound or hypoxia-controlled designs are necessary to avoid masked phenotypes. Match allele to COX histochemistry, monomer to dimer ratios, respirometry, or Leigh-like lesion imaging.
A conventional Ndufa4 knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Ndufa4 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Ndufa4 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Ndufa4-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 251898 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Ndufa4 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Ndufa4 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Ndufa4 Knockout mouse models are available?
When Ndufa4 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Ndufa4 knockout embryonic lethal in mice?
It depends on background and allele design. Some Ndufa4 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Ndufa4 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Ndufa4?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.