Ndufs4 Knockout mouse models
Ndufs4, the mouse ortholog of human NDUFS4, encodes an accessory subunit required for final assembly and stabilization of the complex I N module, the flavin-containing NADH oxidation domain, and it is a substrate for PKA-dependent phosphorylation that tunes holoenzyme stability. Loss does not abolish the enzyme outright but leaves a stalled subcomplex lacking NDUFV1, NDUFV2 and NDUFS6, so NADH oxidation fails while membrane arm subunits persist. Constitutive nulls are viable at birth and then develop progressive ataxia, breathing abnormalities and brainstem and cerebellar lesions, giving one of the most faithful Leigh syndrome models available and a proven platform for interventions such as mTOR inhibition or chronic hypoxia. Conditional floxed alleles let the neuron versus glia versus muscle question be separated, since whole-body loss confounds cell-autonomous interpretation. Choose the conditional null with a defined neuronal Cre for lesion mapping, and reserve the germline null for survival and therapeutic response endpoints.
Ndufs4 null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What Ndufs4 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Ndufs4 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Ndufs4-KO | Knockout | KO/CKO mice, disease model mice | sperm cryopreservation | KO 201311 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Ndufs4 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Ndufs4 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Ndufs4 Knockout mouse models are available?
When Ndufs4 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Ndufs4 knockout embryonic lethal in mice?
It depends on background and allele design. Some Ndufs4 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Ndufs4 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Ndufs4?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.