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Ndufb5 Knockout mouse models
Ndufb5 is the mouse ortholog of human NDUFB5 and encodes the SGDH subunit, a single-pass membrane protein of the complex I membrane arm whose transmembrane helix packs within the ND module while its N-terminal region extends into the matrix, positioning it to stabilize the distal arm during assembly. Loss reduces mature complex I and increases the pool of incomplete intermediates, giving an isolated complex I deficiency signature, and reduced expression has been reported in metabolic and neurodegenerative contexts although a defined human allelic series remains limited. Under those conditions a conditional null is the informative design, with tissue-restricted Cre mapping thresholds in high-demand tissues and inducible Cre separating developmental from maintenance requirements, while a humanized knockin supports functional adjudication of newly identified variants. No paralogous subunit substitutes. Match the allele to endpoints distinguishing quantity from quality of enzyme, including complexome profiling, complex I activity normalized to citrate synthase, respirometry, and superoxide production during reverse electron transport.
A conventional Ndufb5 knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Ndufb5 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Ndufb5 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Ndufb5-KO | KO | KO | DEVELOPING | KO 263130 | Inquire |
| The exon 2-4 of Ndufb5 gene was deleted to generate Ndufb5 knockout mouse. | |||||
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Ndufb5 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Ndufb5 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Ndufb5 Knockout mouse models are available?
When Ndufb5 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Ndufb5 knockout embryonic lethal in mice?
It depends on background and allele design. Some Ndufb5 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Ndufb5 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Ndufb5?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.
Breed this line with ITL
Send the Ndufb5 Knockout line to a U.S. barrier facility for colony maintenance, cohort production, and complex breeding schemes through mouse breeding services.