Pdk1 Knockout mouse models
Nomenclature deserves care here: Pdk1 and human PDK1 denote pyruvate dehydrogenase kinase isozyme 1, a mitochondrial matrix kinase distinct from the PI3K effector Pdpk1. The enzyme phosphorylates serine residues on the E1 alpha subunit of the pyruvate dehydrogenase complex, principally site 1, inactivating the complex and blocking pyruvate entry into the TCA cycle, thereby diverting carbon toward lactate. It is allosterically inhibited by pyruvate and by dichloroacetate and induced transcriptionally by HIF1 alpha, placing it at the switch that installs aerobic glycolysis in hypoxic and tumor tissue. Conditional nulls crossed to cardiomyocyte or tumor-restricted Cre drivers test whether relieving pyruvate dehydrogenase inhibition reverses the glycolytic phenotype, while kinase-dead or hypoxia-response-element mutant knockins isolate catalysis from induction. Because Pdk2, Pdk3 and Pdk4 phosphorylate overlapping sites with different tissue distributions and strong fasting induction, single-isoform deletion frequently yields a masked metabolic phenotype and compound alleles should be planned early.
A conventional Pdk1 knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Pdk1 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Pdk1 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Pdk1-KO | Knockout | KO/CKO mice | embryo cryopreservation | KO 191074 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Pdk1 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Pdk1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Pdk1 Knockout mouse models are available?
When Pdk1 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Pdk1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Pdk1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Pdk1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Pdk1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.