Pdk3 Knockout mouse models
Pdk3, the mouse ortholog of human PDK3, encodes the pyruvate dehydrogenase kinase isoform with the highest intrinsic specific activity toward E1 alpha and the strongest dependence on binding the lipoyl-bearing L2 domain of the E2 core for full activation, a property that renders it comparatively resistant to feedback inhibition by pyruvate. That biochemistry carries clinical weight, since the gene is X-linked and gain-of-function missense variants strengthening L2 binding cause X-linked Charcot-Marie-Tooth neuropathy type 6 through chronic PDH hyperphosphorylation and suppressed oxidative flux. The design fork is therefore unusual for this family: a hyperactivating patient-matched knockin, not a null, reproduces the human disease, while a conditional null answers the separate question of where this isoform normally restrains PDH. Because Pdk1, Pdk2 and Pdk4 overlap substantially, a single knockout is easily buffered and compound alleles should be planned. Match allele to endpoint, pairing peripheral nerve analysis with the knockin and flux measurements with the null.
Pdk3 null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What Pdk3 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Pdk3 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Pdk3-KO | Knockout | KO/CKO mice | embryo cryopreservation | KO 200878 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Pdk3 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Pdk3 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Pdk3 Knockout mouse models are available?
When Pdk3 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Pdk3 knockout embryonic lethal in mice?
It depends on background and allele design. Some Pdk3 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Pdk3 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Pdk3?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.