Pdk4 Knockout mouse models
As the mouse ortholog of human PDK4, Pdk4 encodes the pyruvate dehydrogenase kinase isoform whose control is overwhelmingly transcriptional, induced by fasting, glucocorticoids, PPAR alpha and PPAR delta, FOXO1 and estrogen-related receptors, and repressed by insulin, so its abundance tracks nutrient state rather than acute allosteric input. By phosphorylating E1 alpha during starvation, high fat feeding or diabetes, it conserves pyruvate and lactate for gluconeogenesis while forcing muscle and heart onto fatty acid oxidation, and sustained induction contributes to the metabolic inflexibility seen in insulin resistance and heart failure. That regulatory logic favors a floxed conditional null with muscle, cardiac or hepatic Cre drivers to localize where induction drives glucose oxidation suppression, and a humanized allele where inhibitor pharmacology is the goal. Overlap with Pdk1, Pdk2 and Pdk3 is genuine, so compound conditionals prevent misreading a buffered phenotype. Align the allele with clamp studies, substrate tracing or cardiac efficiency endpoints.
Whole body loss of Pdk4 gives the clearest readout when the question is whether the gene is required at all. Follow up tissue work can still move to a conditional allele if lethality or compensation appears.
What Pdk4 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Pdk4 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Pdk4-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 200879 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Pdk4 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Pdk4 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Pdk4 Knockout mouse models are available?
When Pdk4 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Pdk4 knockout embryonic lethal in mice?
It depends on background and allele design. Some Pdk4 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Pdk4 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Pdk4?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.