Conventional / global / constitutive KO
●3 Models Available
Prkaa1 Conditional Knockout, Knockout mouse models
Browse 3 Prkaa1 mouse models including conditional knockout, knockout variants — in stock and ready to ship from ingenious targeting laboratory. Available as Prkaa1 conditional knockout mouse, Prkaa1 knockout mouse. Request a quote within 24 hours.
Why model Prkaa1
Prkaa1 encodes AMPK alpha1, one of two catalytic subunits orthologous to human PRKAA1, comprising an amino-terminal kinase domain, an autoinhibitory sequence and a carboxy-terminal region that tethers the beta and gamma subunits. Activation requires Thr172 phosphorylation by LKB1 or, upon calcium flux, by CaMKK2, and is sustained allosterically when adenine nucleotide occupancy of the gamma subunit protects that site from phosphatases; downstream, the kinase phosphorylates ACC1 Ser79, TSC2, RAPTOR Ser792 and ULK1 to suppress anabolism and license autophagy. Alpha1 predominates in most tissues except skeletal muscle and liver, where alpha2 dominates, so single deletion often yields a partial phenotype and compound Prkaa1 and Prkaa2 alleles are usually necessary; combined deletion is embryonically lethal, making conditional pairs the practical route. Thr172 phospho-dead knockins separate upstream kinase input from intrinsic catalysis, which matters when interpreting metformin and direct activator studies.
What Prkaa1 mouse models are available?
ingenious targeting laboratory offers 3 distinct Prkaa1 (AMPK) catalog mouse models, featuring conditional knockout and standard knockout. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
| Model Availability | Allele Class Options |
|---|---|
| 3 Ready Catalog Lines | Conditional Knockout (cKO) |
| Custom Model Generation | Constitutive Knockout (KO) |
| Advanced Modifications | Humanized, Knockin, Transgenic |
- Quality Control
- Documented Germline Transmission
- Quality Control
- Strict United States QC Guidelines
- Quality Control
- Cryo or Live Dispatch Options
Prkaa1 model types
PathAvailability
Knockout
Conditional knockout
Knockin
Humanized
Transgenic / overexpression
Prefer the order form? Order. Need an allele that is not listed? Request a quote.
Prkaa1 allele types
Knockout, conditional, knockin, humanized, and related paths for Prkaa1 (human PRKAA1). Catalog lines ship when inventory exists. Everything else is a generation quote.
Knockout
Conditional KO (floxed / loxP)
Inducible conditional KO (CreER / tet)
Tissue specific KO
Specify tissue or Cre driver on quote
Knockout first (tm1a / IKMC)
Convertible floxed allele pathway
Double / compound KO
Multi allele / compound knockout project
Large scale / BAC deletion
BAC scale deletion or targeting
Knockin
Point mutation KI
cDNA KI
Reporter KI (GFP, YFP, RFP, mCherry, tdTomato, lacZ, luciferase)
Tag KI (FLAG, HA, Myc, V5)
Conditional KI (e.g. Rosa26 LSL)
LSL or conditional expression knockin
Gene replacement KI
Humanized
Full gene replacement
Partial / domain humanized
Domain or partial humanization scope
Immune checkpoint humanized
Checkpoint IO humanization when gene is a checkpoint target
Single humanized
Double / multi humanized
Multi humanized / combination IO project
Transgenic
Random integration transgenic
Safe harbor (ROSA26, HPRT, H11, Col1a1)
Overexpression transgenic
BAC transgenic
BAC transgenic or large fragment insert
Cre / recombinase
Constitutive Cre driver
Inducible Cre (CreER / tet)
Tamoxifen or dox inducible Cre
Dual recombinase (Cre+Dre / Cre+Flp)
Dual recombinase breeding scheme
Flp driver
Flp or FRT derivative allele pairing
Reporter
Reporter knockin (gene specific)
Standalone reporter catalog (lineage tracing, constitutive, dual reporter)
Catalog reporter lines not tied to a single gene allele
Species / background
Rat KO / KI / transgenic
Specify rat on quote
Rabbit models
Specify rabbit on quote
C57BL/6 (B6J / B6N)
Background substrain on quote
BALB/c
129, NOD, NSG, FVB, CD1, DBA/2
Background strain on quote
Available Prkaa1 catalog models
3 Prkaa1 lines with QC documentation and technical support. Prkaa1 floxed mice use loxP flanked alleles for Cre dependent tissue specific knockout.
Selected publications from ingenious targeting laboratory
Peer reviewed work involving Prkaa1 (PRKAA1) from ITL supported projects.
- SAPS3 subunit of protein phosphatase 6 is an AMPK inhibitor and controls metabolic homeostasis upon dietary challenge in male mice.Yang Y, Reid MA, Hanse EA, Li H, Li Y, Ruiz BI, Fan Q, Kong M. (2023). Nat Commun 14(1): 1368
- A muscle-specific UBE2O/AMPKα2 axis promotes insulin resistance and metabolic syndrome in obesityVila IK, Park MK, Setijono SR, Yao Y, Kim H, Badin PM, Choi S, Narkar V, Choi SW, Chung J, Moro C, Song SJ, Song MS. (2019). JCI Insight4(13). pii: 128269
- Rho-kinase mediates the anorexigenic action of melanocortin by suppressing AMPKKim SS, Hwang WM, Yang WM, Lee H, Park KS, Dagon Y, Kim YB. (2019). bioRxiv
Looking for a Prkaa1 mouse model?
We have 3 Prkaa1 models in stock — including Conditional Knockout, Knockout types. In stock and ready to ship this week. All models come with full QC documentation, health certificates, and dedicated technical support.
Best pricing in the industry. Get a quote in 24 hours. Our team of PhD scientists is available to help you select the right model for your research.
Order catalog model3
Models Available
2
Model Types
24 hrs
Quote Turnaround
Included
QC Documentation
About Prkaa1 Mouse Model Types
A Prkaa1 knockout mouse has the Prkaa1 gene permanently inactivated, enabling loss of function studies. A Prkaa1 conditional knockout (floxed) mouse carries loxP sites flanking a critical exon of Prkaa1, allowing Cre recombinase dependent deletion in specific tissues or at specific timepoints.
Modifications available
Jump to catalog backed pages for Prkaa1 organized by modification type. Each URL is indexable and matches common search patterns.