Fgf21 Knockout mouse models
Fgf21, the mouse ortholog of human FGF21, encodes an endocrine FGF whose weak heparan sulfate affinity permits circulation and whose activity requires the coreceptor beta-Klotho paired with FGFR1c on adipocytes, or FGFR1c in hypothalamus and hindbrain. Hepatic transcription is induced by PPARalpha during fasting and ketogenesis and by ATF4 during amino acid restriction and integrated stress response, and the protein raises adipose glucose uptake and adiponectin, promotes energy expenditure, and centrally suppresses sweet and alcohol preference while stimulating water intake. Circulating levels are limited by fibroblast activation protein cleavage, a fact exploited by protease-resistant analogs including efruxifermin and pegozafermin. Nulls are viable, so constitutive deletion suits metabolic challenge protocols, while conditional floxed alleles distinguish hepatocyte-derived from adipose or muscle sources and Klb conditionals localize the responsive tissue. No paralog substitutes because beta-Klotho dependence is specific, though FGF15 and FGF19 share the coreceptor with FGFR4. Humanized knockins are the appropriate platform for analog pharmacokinetics and receptor-selectivity endpoints.
Fgf21 null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What Fgf21 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Fgf21 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Fgf21-KO | Knockout | KO/CKO mice | live | KO 190275 | Inquire |
Designing a Fgf21 Knockout allele
Constitutive deletion suits this gene because homozygotes are viable, fertile, and near normal on chow, which makes the allele a challenge-dependent reporter rather than a spontaneous disease model. Remove the whole coding region including the exon encoding the beta-Klotho contact surface, since partial truncations can retain FGFR1c affinity and produce ambiguous partial agonism. The phenotype appears only under stress, so build the protocol before the mouse: ketogenic and low protein diets to test the PPARalpha and ATF4 driven arms, thermoneutral housing to unmask thermogenic contributions, and two-bottle sweet and ethanol tests for the central preference axis. Two caveats matter. Strain background shifts diet responses, so littermate controls on one background are non-negotiable, and a null cannot show whether analogs act through the endogenous axis, which requires Klb deletion. Read ketone bodies, growth under protein restriction, adiponectin, and preference ratios.
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Fgf21 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Fgf21 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Fgf21 Knockout mouse models are available?
When Fgf21 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Fgf21 knockout embryonic lethal in mice?
It depends on background and allele design. Some Fgf21 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Fgf21 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Fgf21?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.