Fgf23 Knockout mouse models
Fgf23, mouse ortholog of human FGF23, encodes the osteocyte and osteoblast derived endocrine regulator of phosphate homeostasis, a ligand with weak heparan sulfate binding that requires alpha-Klotho as coreceptor with FGFR1c on renal proximal tubule and parathyroid cells to suppress the sodium phosphate cotransporters NPT2a and NPT2c and to downregulate CYP27B1 while inducing CYP24A1, thereby lowering serum phosphate and active vitamin D. Bioactivity is set by furin-family cleavage at an RXXR motif that is itself blocked by GALNT3-dependent O-glycosylation, so autosomal dominant hypophosphatemic rickets arises from cleavage-resistant arginine substitutions while familial tumoral calcinosis arises from failed glycosylation or loss of function. Nulls are viable but hyperphosphatemic with ectopic calcification and short lifespan. Allele choice follows disease logic precisely: cleavage-resistant knockins model excess and reproduce XLH-like phenotypes seen with Phex loss, floxed conditionals localize the osteocyte source, and humanized knockins are required for burosumab and other antibody testing against phosphate, ALP, and rickets scoring endpoints.
A conventional Fgf23 knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Fgf23 Knockout mouse models are available?
ingenious targeting laboratory offers 2 distinct Fgf23 knockout catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Fgf23 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Fgf23 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Fgf23 Knockout mouse models are available?
When Fgf23 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Fgf23 knockout embryonic lethal in mice?
It depends on background and allele design. Some Fgf23 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Fgf23 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Fgf23?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.