Fgfr4 Knockout mouse models
Fgfr4, mouse ortholog of human FGFR4, encodes the FGF receptor most distinguished by its endocrine ligand relationship, forming a complex with beta-klotho to serve as the hepatic receptor for FGF19 (mouse Fgf15), which represses CYP7A1 transcription and thereby closes the bile acid feedback loop from ileum to liver. Unlike other family members, FGFR4 undergoes limited receptor internalization and lacks IIIb and IIIc alternative splicing, simplifying allele design. The germline G388R polymorphism enhances STAT3 signaling and associates with cancer progression, FGF19 amplification with FGFR4 dependency defines a hepatocellular carcinoma subset targeted by covalent inhibitors such as fisogatinib and roblitinib, and FGFR4 sustains rhabdomyosarcoma. Null mice are viable with elevated bile acid pool and depleted gallbladder, and only show skeletal phenotypes when combined with Fgfr3 alleles, indicating real but limited redundancy. Use conditional hepatocyte nulls for bile acid and hepatocarcinogenesis questions, and humanized kinase domain knockins when covalent inhibitor cysteine reactivity must translate.
Global Fgfr4 deletion answers broad mechanism questions quickly. If timing or site matters, conditional alleles are a natural next step after the null is characterized.
What Fgfr4 Knockout mouse models are available?
ingenious targeting laboratory offers 2 distinct Fgfr4 knockout catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Fgfr4 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Fgfr4 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Fgfr4 Knockout mouse models are available?
When Fgfr4 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Fgfr4 knockout embryonic lethal in mice?
It depends on background and allele design. Some Fgfr4 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Fgfr4 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Fgfr4?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.