Gdf15 Knockout mouse models
As the most divergent member of the TGF-beta superfamily, the mouse ortholog of human GDF15, Gdf15, produces a circulating hormone that signals not through canonical type II receptors but through GFRAL, a GDNF-receptor-family protein restricted to the area postrema and nucleus tractus solitarius, using RET as its signaling coreceptor. Transcription is driven by the integrated stress response through ATF4 and CHOP, and by p53, which is why plasma levels track mitochondrial disease, metformin exposure, and tumor burden, and why the ligand produces anorexia, nausea-like behavior, and cachexia. Germline nulls are viable, so straight knockouts remain informative, but tissue-restricted conditional deletion is needed to assign hepatic, placental, or tumor sources of the circulating pool. Alternatively, hepatocyte-directed overexpression or humanized knockins allow direct testing of blocking antibodies and long-acting agonists. No close paralog substitutes for this ligand, so single-locus loss is interpretable, and pairing it with Gfral alleles cleanly separates ligand supply from central reception.
Global Gdf15 deletion answers broad mechanism questions quickly. If timing or site matters, conditional alleles are a natural next step after the null is characterized.
What Gdf15 Knockout mouse models are available?
ingenious targeting laboratory offers 2 distinct Gdf15 knockout catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Gdf15 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Gdf15 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Gdf15 Knockout mouse models are available?
When Gdf15 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Gdf15 knockout embryonic lethal in mice?
It depends on background and allele design. Some Gdf15 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Gdf15 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Gdf15?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.