Gdf9 Knockout mouse models
Gdf9, ortholog of human GDF9, encodes an oocyte-restricted TGF-beta ligand that is unusual in signaling through BMPR2 paired with ALK5 to phosphorylate SMAD2 and SMAD3 in granulosa cells, and that forms the potent cumulin heterodimer with BMP15 to amplify cumulus expansion and metabolic support of the oocyte. Follicles arrest at the primary stage without it, so nulls are viable yet female sterile, and human variants cause premature ovarian insufficiency and influence dizygotic twinning rates. Because germline deletion already terminates folliculogenesis, oocyte-specific conditional deletion using Zp3-Cre or Gdf9-Cre is the way to interrogate later antral and periovulatory functions, patient missense knockins separate reduced secretion from impaired heterodimer assembly, and humanization enables recombinant cumulin and antibody assays. Bmp15 is the functional partner rather than a simple backup, and its dosage strongly modifies Gdf9 phenotypes, so compound allelic series are essential for meaningful fertility endpoints.
Gdf9 null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What Gdf9 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Gdf9 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Gdf9-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 240453 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Gdf9 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Gdf9 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Gdf9 Knockout mouse models are available?
When Gdf9 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Gdf9 knockout embryonic lethal in mice?
It depends on background and allele design. Some Gdf9 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Gdf9 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Gdf9?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.