Wnt10a Knockout mouse models
Wnt10a, the mouse ortholog of human WNT10A, encodes a canonical Wnt of the ectodermal appendage program and requires Porcupine-mediated lipidation and Wntless-dependent secretion before signaling through Frizzled with LRP6 to stabilize beta-catenin in dental, hair follicle, nail, and sweat gland epithelium. Human biallelic mutations cause odonto-onycho-dermal dysplasia and Schopf-Schulz-Passarge syndrome, while heterozygous variants produce isolated tooth agenesis, and mouse nulls are viable with cusp and root patterning defects and reduced eccrine gland number, an accessible phenotype set for allele comparison. Conditional deletion resolves epithelial versus mesenchymal requirement across successive appendage stages, patient missense knockins distinguish secretion-defective from binding-defective protein, and humanization supports Porcupine inhibitor and agonist pharmacology. Because Wnt10b and Wnt6 are close paralogs and Wnt6 sits immediately adjacent within a shared regulatory landscape, design floxed alleles that spare neighboring enhancers and plan compound genetics for appendage endpoints.
Whole body loss of Wnt10a gives the clearest readout when the question is whether the gene is required at all. Follow up tissue work can still move to a conditional allele if lethality or compensation appears.
What Wnt10a Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Wnt10a knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Wnt10a-KO | Knockout | KO/CKO mice | embryo cryopreservation | KO 190691 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Wnt10a Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Wnt10a allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Wnt10a Knockout mouse models are available?
When Wnt10a Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Wnt10a knockout embryonic lethal in mice?
It depends on background and allele design. Some Wnt10a germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Wnt10a animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Wnt10a?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.