Wnt2b Knockout mouse models
Wnt2b is the mouse ortholog of human WNT2B and encodes a canonical Wnt that stabilizes beta-catenin through Frizzled with LRP5 or LRP6 in ventral foregut mesenchyme, retinal ciliary margin progenitors, and the mesenchymal compartment supporting intestinal crypt stem cells. Biallelic human loss of function causes early onset chronic diarrhea with intestinal epithelial failure, establishing a non-redundant requirement in the human gut niche that a mouse single null does not obviously reproduce. Conditional deletion in intestinal subepithelial mesenchyme is therefore the informative design for niche questions, patient nonsense or missense knockins model the human enteropathy directly, and humanization supports agonist and R-spondin combination pharmacology. Wnt2 is coexpressed and functionally overlapping, most clearly in lung specification where only compound loss produces agenesis, so compound conditional alleles with a validated mesenchymal Cre driver belong in the initial plan whenever respiratory or intestinal niche output is the endpoint.
Whole body loss of Wnt2b gives the clearest readout when the question is whether the gene is required at all. Follow up tissue work can still move to a conditional allele if lethality or compensation appears.
What Wnt2b Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Wnt2b knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Wnt2b-KO | Knockout | KO/CKO mice | embryo cryopreservation | KO 190692 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Wnt2b Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Wnt2b allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Wnt2b Knockout mouse models are available?
When Wnt2b Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Wnt2b knockout embryonic lethal in mice?
It depends on background and allele design. Some Wnt2b germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Wnt2b animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Wnt2b?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.