Wnt2 Knockout mouse models
Wnt2 is the mouse ortholog of human WNT2 and encodes a canonical ligand of splanchnic mesenchyme that stabilizes beta-catenin through Frizzled with LRP6 in lung mesenchyme, where it specifies Nkx2-1 positive respiratory progenitors from foregut endoderm, and in placental and cardiac progenitor populations. The original null was recognized through placental vascular insufficiency with partial perinatal loss, while lung specification appears grossly intact on its own, which is the key design consideration. Mesenchyme-restricted conditional deletion using Dermo1-Cre or Tbx4 enhancer drivers addresses stage-specific requirements once placental confounding is removed, and humanized alleles support agonist and Porcupine inhibitor pharmacology. Redundancy here is not hypothetical, since Wnt2b is coexpressed in the same ventral foregut mesenchyme and only compound Wnt2 and Wnt2b nulls abolish lung specification and produce lung agenesis. Plan the double conditional and a validated mesenchymal driver from the start rather than after a disappointing single-locus result.
Whole body loss of Wnt2 gives the clearest readout when the question is whether the gene is required at all. Follow up tissue work can still move to a conditional allele if lethality or compensation appears.
What Wnt2 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Wnt2 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Wnt2-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 251105 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Wnt2 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Wnt2 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Wnt2 Knockout mouse models are available?
When Wnt2 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Wnt2 knockout embryonic lethal in mice?
It depends on background and allele design. Some Wnt2 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Wnt2 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Wnt2?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.