Wnt1 Knockout mouse models
Wnt1 is the mouse ortholog of human WNT1 and encodes the founding Wnt ligand, palmitoleated on a conserved serine by Porcupine and exported through Wntless before engaging Frizzled receptors with LRP5 or LRP6 to inhibit the destruction complex and stabilize beta-catenin. Its critical developmental domain is the midbrain and hindbrain organizer, where it maintains an Engrailed, Pax2, and Fgf8 regulatory loop, and the spontaneous swaying allele plus targeted nulls lose midbrain and cerebellum with perinatal death. Human recessive mutations cause osteogenesis imperfecta type XV and heterozygous variants cause early onset osteoporosis, reflecting an osteoblast-autonomous requirement that germline lethality obscures. Conditional deletion is therefore the route to skeletal work, osteogenesis imperfecta missense knockins test secretion versus receptor engagement, and humanization supports pathway agonist testing. Note that Wnt1-Cre is the canonical neural crest driver, so confirm which allele your colony actually carries before interpreting compound crosses.
Wnt1 null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What Wnt1 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Wnt1 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Wnt1-KO | Knockout | KO/CKO mice, disease model mice | sperm cryopreservation | KO 230326 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Wnt1 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Wnt1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Wnt1 Knockout mouse models are available?
When Wnt1 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Wnt1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Wnt1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Wnt1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Wnt1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.