READY TO SHIP!
IN PRODUCTION
Wnt3 Knockout mouse models
Wnt3 is the mouse ortholog of human WNT3 and encodes the canonical ligand that initiates the mouse body axis, acting through Frizzled with LRP5 or LRP6 to stabilize beta-catenin and induce primitive streak formation in the posterior epiblast. Nulls arrest at gastrulation without a streak or mesoderm, an absolute early embryonic lethality that forces conditional and inducible designs for any later question, and human mutations cause tetra-amelia with craniofacial and urogenital malformation, indicating persistent limb induction requirements. Conditional deletion with Sox2-Cre, T-Cre, or tamoxifen-inducible drivers separates epiblast axis initiation from later limb and hair follicle roles, tetra-amelia knockins test whether human variants are secretion defective, and humanization supports pathway agonist testing. Wnt3a cannot substitute at streak initiation despite sharing receptors and acting later in axis elongation, so treat the two as sequential rather than interchangeable and time Cre activity precisely against the streak stage.
A conventional Wnt3 knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Wnt3 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Wnt3 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Wnt3-KO | KO | KO | IN PRODUCTION | KO 262330 | Inquire |
| The exon2 of Wnt3 gene was deleted to generate Wnt3 knockout mouse. | |||||
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Wnt3 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Wnt3 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Wnt3 Knockout mouse models are available?
When Wnt3 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Wnt3 knockout embryonic lethal in mice?
It depends on background and allele design. Some Wnt3 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Wnt3 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Wnt3?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.
Breed this line with ITL
Send the Wnt3 Knockout line to a U.S. barrier facility for colony maintenance, cohort production, and complex breeding schemes through mouse breeding services.