Wnt10b Knockout mouse models
Wnt10b is the mouse ortholog of human WNT10B and encodes a canonical Wnt whose best-defined function is restraining adipogenic commitment, since sustained beta-catenin signaling downstream of Frizzled and LRP5 or LRP6 suppresses PPAR gamma and CEBP alpha induction and holds preadipocytes undifferentiated, while in bone the same ligand supports osteoblastogenesis and trabecular mass. Nulls are viable with reduced trabecular bone and expanded marrow adiposity, and human variants cause split hand foot malformation and tooth agenesis, indicating additional limb and dental appendage roles. Given viability, both conditional deletion and stromal or osteoblast-directed overexpression knockins are practical, the former for cell of origin questions in marrow niches and the latter for testing whether ligand supply alone reallocates lineage output. Humanized alleles support pathway agonist pharmacology. Wnt10a and the adjacent Wnt6 overlap functionally and share regulatory sequence, so preserve neighboring elements when floxing and anticipate compound requirements.
A conventional Wnt10b knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Wnt10b Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Wnt10b knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Wnt10b-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 232052 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Wnt10b Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Wnt10b allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Wnt10b Knockout mouse models are available?
When Wnt10b Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Wnt10b knockout embryonic lethal in mice?
It depends on background and allele design. Some Wnt10b germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Wnt10b animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Wnt10b?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.