Wnt7a Knockout mouse models
Wnt7a is the mouse ortholog of human WNT7A and encodes a canonical Wnt with an unusually well defined patterning role, since dorsal limb ectoderm expression maintains Lmx1b to specify dorsal mesenchyme, so nulls ventralize the dorsal paw and lose dorsal-specific structures, while the same ligand acts through Frizzled 7 and VANGL2 to drive symmetric satellite cell expansion by a planar, mTOR-dependent route, and it directs uterine Hoxa10 and Hoxa11 expression in the female reproductive tract. Human mutations cause Fuhrmann syndrome and the more severe Al-Awadi Raas-Rothschild limb and pelvis hypoplasia, an allelic severity series that motivates precise missense knockins rather than deletion alone. Conditional alleles separate embryonic limb patterning from adult muscle regeneration and endometrial function, and humanization supports agonist and surrogate ligand pharmacology. Wnt7b overlaps substantially in central nervous system angiogenesis and other epithelial contexts, so plan compound conditional genetics for vascular endpoints.
Global Wnt7a deletion answers broad mechanism questions quickly. If timing or site matters, conditional alleles are a natural next step after the null is characterized.
What Wnt7a Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Wnt7a knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Wnt7a-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 190414 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Wnt7a Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Wnt7a allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Wnt7a Knockout mouse models are available?
When Wnt7a Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Wnt7a knockout embryonic lethal in mice?
It depends on background and allele design. Some Wnt7a germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Wnt7a animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Wnt7a?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.