Wnt5a Knockout mouse models
Wnt5a, the mouse ortholog of human WNT5A, encodes the prototypical noncanonical Wnt and signals through Frizzled with the alternative receptors ROR2 and RYK to engage VANGL2, Dishevelled, RHOA, ROCK, and JNK, directing planar polarity and convergent extension while frequently antagonizing beta-catenin-dependent transcription in the same tissue. Nulls die perinatally with severe anterior to posterior truncation, shortened limbs and digits, and craniofacial and genital defects, phenocopying the Ror2 mutant and matching human disease, since heterozygous WNT5A mutations cause dominant Robinow syndrome while recessive ROR2 mutations cause the recessive form. Conditional deletion is therefore required for postnatal work in lung, mammary gland, and tumor stroma, patient cysteine substitution knockins test ligand and receptor coupling rather than absence, and humanization supports pathway pharmacology. Wnt11 and Wnt5b provide overlapping polarity input, so compound conditional alleles and a lineage-validated Cre driver protect against under-reading a real requirement.
Global Wnt5a deletion answers broad mechanism questions quickly. If timing or site matters, conditional alleles are a natural next step after the null is characterized.
What Wnt5a Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Wnt5a knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Wnt5a-KO | Knockout | KO/CKO mice | embryo cryopreservation | KO 210118 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Wnt5a Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Wnt5a allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Wnt5a Knockout mouse models are available?
When Wnt5a Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Wnt5a knockout embryonic lethal in mice?
It depends on background and allele design. Some Wnt5a germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Wnt5a animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Wnt5a?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.