Wnt5b Knockout mouse models
Wnt5b is the mouse ortholog of human WNT5B and encodes the close paralog of WNT5A, sharing its lipidation and secretion requirements and its preference for noncanonical output through Frizzled with ROR2, activating RHOA, JNK, and calcium-dependent responses in growth plate chondrocytes, adipocyte progenitors, pancreatic islets, and digit mesenchyme. Nulls are grossly normal, which is the central design fact, because the informative experiment is almost never single-locus deletion but compound Wnt5a and Wnt5b genetics, where dosage reduction aggravates chondrocyte column disorganization and limb shortening beyond either single mutant. Accordingly, build a conditional Wnt5b allele intended for use on a Wnt5a-sensitized background, add overexpression knockins to test whether Wnt5b can substitute for Wnt5a when supplied in the correct domain, and humanize the ligand for receptor selectivity pharmacology. Pair these with a chondrocyte or limb mesenchyme driver, since interpretable skeletal endpoints depend on restricting deletion to the relevant lineage.
A conventional Wnt5b knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Wnt5b Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Wnt5b knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Wnt5b-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 240964 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Wnt5b Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Wnt5b allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Wnt5b Knockout mouse models are available?
When Wnt5b Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Wnt5b knockout embryonic lethal in mice?
It depends on background and allele design. Some Wnt5b germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Wnt5b animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Wnt5b?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.