Wnt9a Knockout mouse models
Wnt9a is the mouse ortholog of human WNT9A and encodes a canonical Wnt that stabilizes beta-catenin through Frizzled with LRP5 or LRP6 and is the principal inducer of synovial joint identity, since ectopic expression converts cartilage toward joint interzone fate while loss permits ectopic chondrogenesis within joints and joint fusion. Beyond the skeleton it acts from stromal niches to support hematopoietic stem cell function and from ureteric epithelium to help initiate the mesenchymal to epithelial transition of nephron progenitors, and nulls die perinatally with renal hypoplasia. Joint-restricted conditional deletion using Gdf5-Cre isolates articular maintenance from renal lethality, gain-of-function knockins test the joint induction model directly, and humanization supports agonist pharmacology, while human WNT9A variants in autopod malformation motivate patient missense alleles. Wnt9b overlaps in kidney and duct programs, so compound conditional alleles are advisable whenever urogenital rather than articular endpoints are primary.
Whole body loss of Wnt9a gives the clearest readout when the question is whether the gene is required at all. Follow up tissue work can still move to a conditional allele if lethality or compensation appears.
What Wnt9a Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Wnt9a knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Wnt9a-KO | Knockout | KO/CKO mice | embryo cryopreservation | KO 232367 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Wnt9a Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Wnt9a allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Wnt9a Knockout mouse models are available?
When Wnt9a Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Wnt9a knockout embryonic lethal in mice?
It depends on background and allele design. Some Wnt9a germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Wnt9a animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Wnt9a?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.